@article { author = {Waghmare, Pankajkumar R. and Khandare, Rahul V. and Jeon, Byong-Hun and Govindwar, Sanjay P.}, title = {Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production}, journal = {Biofuel Research Journal}, volume = {5}, number = {3}, pages = {846-853}, year = {2018}, publisher = {Alpha Creation Enterprise}, issn = {2292-8782}, eissn = {2292-8782}, doi = {10.18331/BRJ2018.5.3.4}, abstract = {Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus Phanerochaete chrysosporium (MTCC 4955) under submerged static condition. Ligninolytic enzymes like lignin peroxidase (0.843 U/mL) and manganese peroxidase (0.389 U/mL) played an important role in the biological pretreatment of sorghum husk. Activities of different hydrolytic enzymes such as endoglucanase (57.25 U/mL), exoglucanase (4.76 U/mL), filter paperase (0.580 U/mL), glucoamylase (153.38 U/mL), and xylanase (88.14 U/mL) during biological pretreatment of sorghum husk by P. chrysosporium were evaluated. Enzymatic hydrolysis of untreated sorghum husk and biologically pretreated sorghum husk produced 20.07 and 103.0 mg/g reducing sugars, respectively. This result showed a significant increase in reducing sugar production in the biologically pretreated sorghum husk as compared to its untreated counterpart. Biologically pretreated sorghum husk hydrolysate was further fermented for 48 h using Saccharomyces cerevisiae (KCTC 7296), Pachysolen tannophilus (MTCC 1077), and their co-culture resulting in ethanol yields of 2.113, 1.095, and 2.348%, respectively. The surface characteristics of the substrate were evaluated after the delignification and hydrolysis, using FTIR, XRD, and SEM, confirming the effectiveness of the biological pretreatment process.}, keywords = {Sorghum husk,Phanerochaete chrysosporium,Delignification,Biological pretreatment,Enzymatic hydrolysis,Ethanol production}, url = {https://www.biofueljournal.com/article_68378.html}, eprint = {https://www.biofueljournal.com/article_68378_3e65242df039dc49815df6fdf811c0b0.pdf} }