TY - JOUR ID - 15101 TI - Maximising high solid loading enzymatic saccharification yield from acid-catalysed hydrothermally-pretreated brewers spent grain JO - Biofuel Research Journal JA - BRJ LA - en SN - AU - Wilkinson, Stuart AU - Smart, Katherine A. AU - James, Sue AU - Cook, David J. AD - Brewing Science Section, Division of Food Sciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, U.K. AD - SABMiller Plc, SABMiller House, Church Street West, Woking, Surrey, GU21 6HS, U.K. Y1 - 2016 PY - 2016 VL - 3 IS - 2 SP - 417 EP - 429 KW - Brewers Spent Grains KW - Bioethanol KW - Enzymatic saccharification KW - High solids loading DO - 10.18331/BRJ2016.3.2.7 N2 - Enzyme saccharification of pretreated brewers spent grains (BSG) was investigated, aiming at maximising glucose production. Factors investigated were; variation of the solids loadings at different cellulolytic enzyme doses, reaction time, higher energy mixing methods, supplementation of the cellulolytic enzymes with additional enzymes (and cofactors) and use of fed-batch methods. Improved slurry agitation through aerated high-torque mixing offered small but significant enhancements in glucose yields (to 53 ± 2.9 g/L and 45% of theoretical yield) compared to only 41 ± 4.0 g/L and 39% of theoretical yield for standard shaking methods (at 15% w/v solids loading). Supplementation of the cellulolytic enzymes with additional enzymes (acetyl xylan esterases, ferulic acid esterases and α-L- arabinofuranosidases) also boosted achieved glucose yields to 58 – 69 ± 0.8 - 6.2 g/L which equated to 52 - 58% of theoretical yield. Fed-batch methods also enhanced glucose yields (to 58 ± 2.2 g/L and 35% of theoretical yield at 25% w/v solids loading) compared to non-fed-batch methods. From these investigations a novel enzymatic saccharification method was developed (using enhanced mixing, a fed-batch approach and additional carbohydrate degrading enzymes) which further increased glucose yields to 78 ± 4.1 g/L and 43% of theoretical yield when operating at high solids loading (25% w/v). UR - https://www.biofueljournal.com/article_15101.html L1 - https://www.biofueljournal.com/article_15101_f7f2e8ff5a52f4d939e420a6884d6111.pdf ER -