Lipase immobilized on polydopamine-coated magnetite nanoparticles for biodiesel production from soybean oil

Document Type: Research Paper

Authors

Instituto de Química, Universidade de São Paulo, 05508-000, São Paulo, Brazil

Abstract

Lipase from Pseudomonas cepacia was covalently attached to magnetite nanoparticles coated with a thin polydopamine film, and employed in the enzymatic conversion of soybean oil into biodiesel, in the presence of methanol.  The proposed strategy explored the direct immobilization of the enzyme via Michael addition and aldolic condensation reactions at the catechol rings, with no need of using specific coupling agents. In addition, a larger amount of enzymes could be bound to the magnetic nanoparticles, allowing their efficient recycling with the use of an external magnet. In the biodiesel conversion, the transesterification reaction was carried out directly in soybean oil by the stepwise addition of methanol, in order to circumvent its inactivation effect on the enzyme. A better yield was  obtained in relation to the free enzyme, achieving 90% yield at 37 oC.  However, the catalysis became  gradually less effective after the third cycle, due to its prolonged exposition to the denaturating methanol medium.

Graphical Abstract

Lipase immobilized on polydopamine-coated magnetite nanoparticles for biodiesel production from soybean oil

Highlights

  • Lipase attached to polydopamine magnetite nanoparticle converted soybean oil into biodiesel with high efficiency.

  • The polydopamine film allowed direct binding of the enzyme.

  • Polydopamine also promoted immobilization of a large amount of enzymes onto the magnetic nanoparticles.

  • The enzyme could be magnetically recycled.

Keywords