Enhanced ethanol and glucosamine production from rice husk by NAOH pretreatment and fermentation by fungus Mucor hiemalis

Document Type: Research Paper

Authors

1 Department of Chemical Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.

2 Industrial Biotechnology Group, Institute for Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.

Abstract

Ethanol production from rice husk by simultaneous saccharification and fermentation using Mucor hiemalis was investigated. To reach the maximum ethanol production yield, the most important influencing factors in the pretreatment process, including temperature (0-100°C), NaOH concentration (1-3 M), and the pretreatment time (30-180 min), were optimized using an experimental design by a response surface methodology (RSM). The maximum ethanol production yield of 86.7 % was obtained after fungal cultivation on the husk pretreated with 2.6 M NaOH at 67°C for 150 min. This was higher than the yield of 57.7% obtained using Saccharomyces cerevisiae as control. Furthermore, fermentation using M. hiemalis under the optimum conditions led to the production of a highly valuable fungal biomass, containing 60 g glucosamine (GlcN), 410 g protein, and 160 g fungal oil per each kg of the fungal biomass.

Graphical Abstract

Enhanced ethanol and glucosamine production from rice husk by NAOH pretreatment and fermentation by fungus Mucor hiemalis

Highlights

  • Ethanol production from rice husk using Mucor hiemalis was investigated.
  • The maximum ethanol production yield of 86.7% was observed after pretreatment with 2.6 M NaOH at 67°C for 150 min.
  • A highly valuable fungal biomass containing 60 g/kg glucosamine was obtained at optimum conditions.

Keywords